Dynamic morphology and cytoskeletal protein changes during spontaneous inside-out vesiculation of red blood cell membranes
نویسندگان
چکیده
منابع مشابه
Mechanism of spontaneous inside-out vesiculation of red cell membranes
In certain conditions, human red cell membranes spontaneously form inside out vesicles within 20 min after hypotonic lysis. Study of the geometry of this process now reveals that, contrary to earlier views of vesiculation by endocytosis or by the mechanical shearing of cytoskeleton-depleted membrane, lysis generates a persistent membrane edge which spontaneously curls, cuts, and splices the mem...
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The relationship between active extrusion of Ca(++) from red cell ghosts and active uptake of Ca(++) by isolated red cell membrane fragments was investigated by studying the Ca(++) uptake activities of inside-out and right side-out vesicles. Preparations A and B which had mainly inside-out and right side-out vesicles, respectively, were isolated from red cell membranes and were compared with re...
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Shapes of red blood cells at low pH were studied theoretically. It is assumed that the equilibrium shape of the red blood cell corresponds to the minimum of its membrane elastic energy which consists of the bending energy and relative stretching energy of the bilayer, the stretching energy of the skeleton and the interaction energy between the skeleton and the bilayer. It is shown that the aggr...
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We employ a novel optical technique, dynamic scattering microscopy (DSM), to extract the frequency dependence of the viscoelastic modulus associated with the red blood cell membrane. This approach applies the principle of dynamic light scattering to micro beads attached to the red blood cell membrane in thermal fluctuation. This allows for highthroughput characterization of a large number of ce...
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Hereditary hemolytic anemia encompasses a heterogeneous group of anemias characterized by decreased red blood cell survival because of inherited membrane, enzyme, or hemoglobin disorders. Affected red blood cells are more fragile, less deformable, and more susceptible to shear stress and oxidative damage, and show increased vesiculation. Red blood cells, as essentially all cells, constitutively...
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ژورنال
عنوان ژورنال: Pflügers Archiv - European Journal of Physiology
سال: 2014
ISSN: 0031-6768,1432-2013
DOI: 10.1007/s00424-014-1483-5